All users must read this entire manual to fully understand the safe use of Storm. 15 and by a newly developed computer-aided analysis procedure. 0 for Microsoft Windows 375-635. Lanes 5 and 7 use the BYDV luciferase transcript,. DIGE gel image was scanned at 100 μm resolution on Typhoon 9410 variable mode imager (GE Healthcare) using excitation/emission wavelengths specific for Cy2 (488/520 nm, blue laser), Cy3 (532/580 nm, green laser) and Cy5 (633/670 nm, red laser). before loading, always make sure to place the manual hammer stop in the "on" position, and the hammer into the safety (rebound), position. 1 software (Bio-Rad, USA).
Lanes 1 and 3 use the BYDV HaloTag™ transcript, and lanes 2 and 4 use the poly(A) HaloTag™ transcript. E-mail Promega Technical Services if you have questions on use of this system: com pHAb Amine and Thiol Reactive Dyes 1. automatically activated. The Typhoon Trio instrument contains three internal lasers (blue, green, and red). Visit the web site to verify that you are using the most current version of this Technical Manual. Our quality controls and manufacturing procedures are designed to help deliver consistent results whether your concern is over one laser or a thousand. visualized by Typhoon 9410 Imager (GE Healthcare), which was set at the appropriate wavelengths for each dye. Molecular Dynamics Typhoon 9410 Variable Mode Imager System.
the Typhoon™ 9410 workstation. Positively hybridizing DNA bands were visualized by overnight exposure of the blot with a Phosphor screen and scanned by a Typhoon 9410 variable mode imager (Molecular Dynamics, Amersham Pharmacia Biotech, Piscataway, NJ, USA). Prior to usage customers may contact the Genomics Core to arrange instrument training although the supplied manual is very. The safety interlocks in the Typhoon instrument are designed to prevent you from being. 2, GE Healthcare) batch processor for Differential-In gel Ana-.
Unusually, the erythromycin biosynthetic ( ery ) cluster lacks a pathway-specific regulatory gene. user manual um Typhoon User’s Guide v3. Blue Laser Module Laptop w/ Image Quant and Scan Control Software loaded on computer All Necessary Cables, Cords and Manuals Powerful excitation sources and innovative high-quality confocal optics allow for the s. Fluorescent intensities of scanned 2D images were analyzed using PDQUEST 8. While ZapA has the ability to degrade host immunoglobulins (Igs), the dramatic attenuation of virulence in ZapA mutants suggests that this enzyme may have a broader spectrum of activity. IPGphor user manual, and 2-D electrophoresis. Computational analysis of seroreactivity patterns Since manual inspection offers only a subjective and. The Typhoon 9410 exhibits outstanding linearity quantitative accuracy and extremely low limits of detection.
GE Typhoon Variable Mode Scanner. caution: always make sure the shells are of the proper gauge and length for your model 9410 shotgun. 5% SDS, for 30 min and in 1× SSC, 0. Choose from four different Typhoon models depending on your imaging needs. The 54-kDa extracellular metalloprotease ZapA is an important virulence factor of uropathogenic Proteus mirabilis. Typhoon 9410 scans gels blots and mounted or unmounted storage phosphor screens up to 35 × 43 cm.
the ammunition designation on the barrel. Autoradiography was performed with a Typhoon 9410 variable Mode Imager (GE Amersham Biosciences, Piscataway, NJ, USA). · Saccharopolyspora erythraea makes erythromycin, an antibiotic commonly used in human medicine. The Typhoon 9410 can scan at pixel sizes of 10, 25, 50, 100, 200, 500, and 1000 μm. This manual is available in the following languages: English. Scanning the plate gives a visual readout of fluorescence, and allows manual exclusion of wells with clumped cells or other artefacts.
· For all gels, imaging was done using a Typhoon 9410 image system equipped with a 457 nm laser and 580 nm filterset. Laser power was chosen so that no saturated signal was obtained from any protein spot. Free shipping on many items | Browse your favorite brands | affordable prices. The albumin spot was excluded from the matchset due to intensity saturation. Get the best deals on Amersham Biosciences Other Medical & Lab Equipment when you shop the largest online selection at eBay. All typhoon 9410 manual gels were scanned at 100μm resolution and the photomultiplier tube was set between 5V. This manual comes under the category Sewing machines and has been rated by 5 people with an average of a 7.
For DNA, RNA, and protein samples, choose from: storage phosphor autoradiography, direct blue, green or red excited fluorescence. and scanned in a Typhoon 9410 (GE Healthcare). National Laser has established a reputation of improving upon original design in order to advance laser performance and lifetimes. Typhoon 9410 scans gels blots and mounted or un-mounted storage phosphor screens up to 35 × 43 cm. Typhoon 9410 GE Healthcare.
Guaranteed for life. Location: Porter Building (Bldg. The Typhoon instrument is a variable-mode imager that produces digital images of radioactive, fluorescent, or chemiluminescent samples. See more results. When one of the scanning modes is selected the Typhoon 9410 activates the appropriate optical components are automatically. Ask your question here.
This hypothesis was tested by in vitro assays with purified ZapA and an array of purified. 2472 ª The Authors. TYPHOON F12 OWNERS MANUAL: TYPHOON XII OWNERS MANUAL: TYPHOON K12 OWNERS MANUAL: KINETIC OWNERS MANUAL.
1 MB · Views: 214 Typhoon-H-compass-calibration. · Dell Latitude 9410 Notebook System Guide View Page This article is a collection of all of the articles relevant to the Dell Latitude 9410 2-in-1 Notebook as it ships from Dell. View the manual for the Singer 9410 here, for free. The evaluation of the scanned protein arrays was carried out by the image ana-lyzing software tool AIDA version 4. sign highlights instruc-tions that must be followed to avoid per-sonal injury. We isolated a transcriptional regulator of the ery biosynthetic genes from S. In-gel trypsin digestion and MALDI peptide mass fingerprinting (PMF) was performed as previously described 46 with slight modifications (mass tolerance value was 150 ppm and E. Images were then exported to DeCyder (v 7.
Internationalstandards Thisproductfulfillstherequirementsofthefollowingstandards: Standard Description Notes EN61010-1 harmonized with /95/EC. Construction of NGRΩnopL-nopL(S12xA). To speak with a representative, please fill out the contact form below or call us directly. Stained gels were scanned using a Typhoon 9410 imager using a 532 nm laser and a 610 nm BP30 emission filter.
Where indicated, gels were stained with CBB (Coomassie Brilliant Blue G-250). Western Blotting Overview 2–3 Western Blotting 10 Steps Overview 4–5 Step 1 – Gel Electrophoresis Protein Gel Accessories 6 Protein Ladders 7–12. Thank you for your interest in the Molecular Dynamics Typhoon 9410 Variable Mode Imager System (ID : 14615). Manual editing of spots was performed when needed. push the last shell fully in, allowing the cover to close. The membrane was washed in 6x SSC at room temperature for 2 min.
When repairing the laser, National Laser replaces the existing laser tube with an entirely new laser tube. 35) Room 3A1014 Laser lines: 457, 477, 488, 532, 633 Applications: The Typhoon 9410 Variable Mode Imager typhoon 9410 manual is a versatile multi- purpose laser scanner. Lane 9 is Wheat Germ Extract Plus without mRNA. 9410L Flashlight rechargeable flashlight Super bright flashlight, offering waterproof water resistance and brightest LEDs. Do you have a question about the Singer 9410 or do you need help? 5 KB · Views: 123. © Typhoon Defence Silah Makina Oto.
Typhoon Variable Mode Imager exhibits outstanding linearity and quantitative accuracy, and includes ImageQuant™ Image Analysis Software for Windows™. Typhoon scans mounted and unmounted storage phosphor screens, plus gels and blots up to 35 × 43 cm as well as microarray slides for Typhoon 92 models. Lanes 1, 2, 5, and 6 are Wheat Germ Extract Plus and lanes 3, 4, 7, and typhoon 9410 manual 8 are Wheat Germ Extract. The blue laser, with power at 30 mW, is accessible in the interior of the module. Typhoon is intended fo r laboratory use only, not for clinical or in vitro use, or for diagnostic purposes.
The corresponding pixel numbers per well and total scanning times for forty-five 384-well plates containing the MGC cDNA library are listed in Table 1. Includes: Red, Blue, Green Laser Modules Built in. It is important not to.
· Rather than using a microplate reader, as the manufacturer recommends, we scan the 96-well plate on a GE Healthcare Typhoon 9410 scanner, and quantify fluorescence intensity per well using ImageQuant software. The Typhoon instrument is available in several models. Typhoon 9400, 9410, Trio, and Trio+: The blue laser module contains one Class IIIB laser. 5% SDS, and then analyzed using a phosphorimage screen and a Typhoon 9410 scanner (GE Healthcare). ge amersham typhoon 9410 lab variable mode imager molecular scanner+blue laser. erythraea and found that this protein appears to directly link morphological changes caused by impending starvation to the.
The hybridized membrane was washed twice for 30 min in 2× SCC, 0. 4 KB · Views: 115 Typhoon-H-sheets.
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